FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004914746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31342677
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT3819591.2186546796880178No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG3184211.015934280278612No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT2085210.6652941610571427No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC1993140.6359188782757772No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1067010.34043358836260224No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC948360.30257785574601687No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC789130.25177492018311004No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA655370.2090982847444716No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG641610.20470810454384608No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA633200.20202486213924867No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG480890.15342977882840064No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT470390.1500797139950745No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC463410.14785271851539675No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA442790.14127382929033153No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG422940.13494061148637687No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA419310.13378244621542698No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC405850.12948798215289653No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG395640.1262304429197289No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG366630.11697469236593926No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG359140.11458497945149994No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT358840.11448926331340491No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA350250.11174859122595047No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG346100.11042451798230254No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC343180.10949288090484421No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC336830.10746688931516603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)