FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9399381
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1295171.377931163764933No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG998811.062633805353778No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT685050.7288245896192526No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC562970.5989436963987309No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC241030.2564317799225289No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA228680.24329261682232056No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA211250.2247488425035649No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG204820.21790796649268715No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC190830.20302400764475875No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC161680.1720113271288822No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA149960.15954242093176135No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA141090.15010562929622706No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG136830.14557341595153978No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC133110.14161570852378472No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG124390.132338501865176No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT119820.12747647956817582No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA112160.1193270067465081No Hit
ACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAA101800.10830500434017942No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG97700.10394301497087946No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC95340.10143221133391656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCCG227000.066.31311
CGACTCC237400.065.661336
ACTCCCT244050.063.906998
CCGACTC243050.063.0800365
GACTCCC253400.061.57167
AACCCGA255800.059.1943242
ACCCGAC257700.058.8882183
CCCGACT265450.058.072654
CTCCCTT269350.058.045239
TTGGACG45550.054.3086287
GTAGCAA81150.046.8051079
AGTAGCA83300.045.6540538
GCGACAT12550.043.1301165
TAGTAGC88100.042.899947
GAGTAGT47000.039.013471
TGCGACA14150.038.9928554
TAGAGCC81500.038.1595467
TTAGAGC83600.037.14426
GCTTGGA68850.036.4124155
AGTAGTG53000.035.5762632
Produced by FastQC (version 0.11.5)