FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728692
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACNCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG49980.2891203291274559No Hit
TGGTNGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG40750.23572735918254958No Hit
GAACNCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT24620.1424198179895551No Hit
AGCCNTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC22440.12980912736334754No Hit
GTATNCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA20030.11586795102887038No Hit
CTCANGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT18290.10580253740978729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTA29350.068.428245
CCGACTC14850.065.76075
TATTCGT11450.065.445982
GAACCCG14850.063.716881
CCCGACT15750.063.172814
GGTGGTA30300.062.0308042
TGGTAGT30700.061.8181234
CGACTCC16600.060.471476
GTAGCAA41350.060.3505069
TAGTAGC40900.060.211697
TAGAGCC17450.058.869937
GTGGTAG32450.058.8628653
ACCCGAC17050.057.6356663
AGTAGCA43550.057.5172278
GTAGTAG37600.057.2624176
TGGTGGT31650.057.0734181
AACCCGA16750.056.8388822
TTCGTAC13350.056.587384
ATTCGTA13300.056.338323
TTAGAGC18700.055.436466
Produced by FastQC (version 0.11.5)