FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728692
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCA13750.077.507198
TAGTAGC13350.072.770117
GTAGCAA17350.063.2732969
GGTAGTA8350.061.245485
GTAGTAG11050.060.153626
GTACACC5600.059.347997
ACGCATT3800.057.1960263
TACGCAT3200.057.1622962
CGCATTT4800.055.9343454
GGTACAC5050.055.6867646
CGGTACA5100.055.150955
CATTGCG2750.053.824448
GCGTACA1457.092294E-752.9034234
ACGAGAA13300.050.5183424-25
CACCGTC3600.050.1274769
CGCGTAC800.00994987547.9437263
TGTCGGC8550.047.8414767
CGAGAAC12700.047.20269824-25
TCCGTTT4000.044.7474823
GTCGGCT9050.044.705018
Produced by FastQC (version 0.11.5)