FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10857343
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1021730.9410497577538077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT502230.4625717360131295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370570.3413081819373303No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162940.1500735493020714No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA138230.12731475831609998No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG128210.11808598107290154No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG126190.11622548905381362No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA124950.11508340484407649No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG121880.11225582538932408No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC114510.10546779262661224No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG111490.10268626495451051No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG110710.10196785714515974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123700.091.267781
GCGTACT4450.073.556751
GTATCAA396200.068.917381
GCAGCGC23650.065.411198
ATCAACG468900.057.989423
GCTCTAG24900.057.841011
TGCAGCG26600.057.7109077
TCAACGC477450.057.2241364
CAACGCA476950.057.2092635
AACGCAG502500.054.3858876
CGTACTT6400.051.1449242
ACGCAGA540500.050.5505647
CGCAGAG545150.050.1073158
TATCAAC569050.047.690762
GCAGAGT583500.046.844659
GCGTAAG6300.046.2887461
TCTGTCG15700.043.2031178
CGTAACG1256.2391337E-1042.850152
GCGTAAC2400.042.1558231
CATGCAG41500.037.8578265
Produced by FastQC (version 0.11.5)