FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10857343
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA134840.12419244745238314No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA122350.11268871214624057No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG117250.10799143031587011No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG111580.10276915816328175No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC110580.10184812251026795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGC21300.061.4571848
TGCAGCG24000.054.5389757
GCTCTAG23550.050.5870671
CAACGCA62100.043.7731675
CGCAGAG62800.042.636598
ACGCAGA63750.042.464577
GGTATCA30250.041.5487061
ATCAACG69350.039.0346643
TCAACGC69100.038.9905624
CATGCAG39950.034.0958985
AACGCAG81050.033.6189356
GCAGAGT82150.032.961849
GTCGCGC953.2667376E-431.3506681
GTCTCGC17300.030.9882341
GTATCAA99150.029.6179261
TCTGTCG15000.028.164158
CTCGCTC16150.027.9979723
TCTCGCT17750.027.4853322
TGGTCCT36650.026.292055
GAGTACG74450.024.46326812-13
Produced by FastQC (version 0.11.5)