FastQCFastQC Report
Fri 10 Feb 2023
EGAF00004914761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86048521
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA4987430.5796067081734038No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG4505650.5236173669969295No Hit
GGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGC2949410.34276126605360246No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA2763600.32116763517643726No Hit
GGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGG2736450.31801243858682937No Hit
GGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGC2666470.30987981769030054No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG2658730.30898032518188195No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG2288780.26598713997652557No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA2195960.25520020268564525No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCG2154260.25035409963641325No Hit
GGTGGTTCCGCATGGAAGGGCCATCGCTCAACGGATAAAAGCTACCCCGG2062810.23972637484379308No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1981750.23030610834089757No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG1971350.2290974879161491No Hit
GCGCCGAAAATGTACCGGGGCTAAACGTATCACCGAAGCTGCGGACTGTT1889990.21964235736253968No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC1858380.21596884855231854No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1522650.1769524893983942No Hit
GCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACCGAATGCCT1176800.13676004960038768No Hit
GGGCATTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT1144210.13297265155783444No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG1139830.13246363641741152No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1126270.13088778132514328No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC1126220.1308819706500243No Hit
GCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCTAGTACGAGAGGACC1111600.1291829292452336No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGA1003410.11660979042277786No Hit
TGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGG989560.11500023341481952No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA983800.11433084364111266No Hit
GGGCGCTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT975180.11332908325059998No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG974830.1132884085247671No Hit
CCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGT974760.11328027357960051No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG973360.11311757467626898No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC947980.11016807598587314No Hit
GGGCCCTGTCTCAACGAGAGACTCGGTGAAATTATAGTACCTGTGAAGAT946050.10994378392628038No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA940730.10932552809362057No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT940510.10929996112309705No Hit
GTAGTCGAAAGGGAAACAGCCCAGACCGCCAGCTAAGGTCCCAAAGTATA906770.10537891755280722No Hit
CGTGCAAGGTTAAGCAGAAGATGCGGAGCCGCAGCGAAAGCGAGTCTGAA874670.10164846412642002No Hit
GGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGCACGAAGGTTGGCTAAT866650.10071643183733511No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)