FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10871247
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1016880.9353848735108309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT496680.4568749104863499No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371380.34161674369094913No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160820.1479315114448232No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA139080.12793380556986703No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG126800.11663795330931216No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA126040.11593886147559704No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG124870.11486262799474616No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG120940.1112475873282982No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC115130.10590321423108132No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG113640.1045326262939293No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG110820.10193862764777582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125500.090.84551
GTATCAA395050.068.414271
GCGTACT4400.060.8571321
GCGTAAG5700.059.504751
ATCAACG470450.057.3716353
GCAGCGC24650.056.9650848
CAACGCA480500.056.3447725
TCAACGC482850.056.1198434
AACGCAG503300.053.7661826
GCTCTAG23500.053.1744581
TGCAGCG27900.050.329377
ACGCAGA543000.049.8130657
CGCAGAG546800.049.456018
TATCAAC575750.046.766222
CGTACTT5600.046.75332
GCAGAGT584900.046.2548269
TCTGTCG17150.039.8976558
CGTACCG1053.2653224E-739.6694682
GCGTAAC1551.2187229E-1038.390161
GTCTCGC15700.037.9011151
Produced by FastQC (version 0.11.5)