FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10871247
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA137030.12604809733418806No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA120210.11057609122486131No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG116690.10733819220554919No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG112480.1034655913898378No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC109280.10052204682682678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGC20150.055.2078868
GCTCTAG24550.049.004341
TGCAGCG23700.046.9372677
CAACGCA59200.045.239875
ACGCAGA61950.043.4031947
CGCAGAG62150.043.073088
GGTATCA30250.041.542411
TCAACGC68050.039.356184
ATCAACG69200.039.0472343
TCTGTCG16100.037.68858
GCAGAGT78400.034.0725679
GTCTCGC13900.033.8490871
AACGCAG79150.033.8206636
GTATCAA94300.030.568091
CTCGCTC15100.029.9555423
CATGCAG38550.029.1788355
TCTCGCT15950.029.1049212
TGGTCCT37950.028.3855515
GAGTACG72200.024.80594812-13
CCGTATG26300.024.4310729
Produced by FastQC (version 0.11.5)