FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9632721
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC125640.13043043601075957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT25150.054.3907245
TGCGACA25350.053.963854
CTGCGAC31600.043.7420163
CGACGCT61500.041.627428
GCTGCGA33100.041.188942
GGTGTGC118000.040.6137167
GACGCTC64900.038.6415949
CGACATC35550.038.3451466
GCGACGC31450.037.1516467
CCGACGC35550.035.6726387
GTGTGCT169200.035.5385068
TGCGACG12600.034.68056
TGTGCTC130100.033.9524779
GGCTGCG43600.032.061051
GTGCGAC11350.031.3859845
TCGACGC8200.028.3821817
TCCGACG12050.026.0150666
CGCTCTT55350.025.44333510-11
GCCGACG16650.024.818366
CGGTGTG41550.024.6916896
Produced by FastQC (version 0.11.5)