FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8365268
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG746910.8928703778528077No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT388270.46414532086718563No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT386100.461551261716899No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC379940.454187480903182No Hit
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAA261720.3128650510659073No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC236690.28294371441536603No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG156260.18679616719990322No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG155440.18581592365002533No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC145170.17353897089728626No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG143150.1711242245914895No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA136650.16335400133026223No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA129140.15437640491613658No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA125960.15057497261295155No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA119380.14270911583466303No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCG112360.1343172747125376No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT103370.12357045823277867No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC101100.12085685718616547No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG99190.11857360696632792No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG88130.10535227323260891No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC87030.10403731237301661No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT84490.10100094820632166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG12650.045.4812161
GTAGCAA84500.043.788059
CGACTCC100850.043.3290336
CCGACTC102750.042.5742955
AGTAGCA88250.041.819728
GACTCCC105400.041.368447
ACTCCCT105850.041.2830548
TAGTAGC91300.040.2138637
GAACCCG109550.039.7142071
GCGACAT8250.038.573635
AACCCGA116500.037.4703522
CCCGACT120150.037.1620524
CGGACCA80450.036.250335
GTAGTAG81700.035.986176
CACGGAC81800.035.8866923
CTCCCTT123700.035.05719
ACCCGAC126250.034.802353
ACACGGA85400.034.262662
TAGAGCC80550.033.6695757
TGCGACA9550.033.3247644
Produced by FastQC (version 0.11.5)