FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437911
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198060.576105664166408No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT102730.29881518166118903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91310.26559733512589473No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA90560.26341577777900593No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC89580.26056520951240447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89430.2601288980430267No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT72510.2109129642972142No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC60820.17690975711703996No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC59830.17403010141914668No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC59670.17356470251847705No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT57840.16824170259206825No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT51850.1508183312482493No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA49940.1452626318715057No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA49190.14308107452461685No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC46590.1355183423887355No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC45730.13301682329763626No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC45660.1328132112785933No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT44810.13034077961878596No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG40080.11658242461774024No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC39860.11594250112931953No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT37400.10878699303152409No Hit
CTCTATACGGAGTTACAAAGGAACGAGGTAAATGAACAGGTCTGGAAAGG34800.10122426089564274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA346050.056.319981
TATCAAC474850.041.1896972
ATCAACG496650.039.309853
TCAACGC509900.038.3816954
CAACGCA535850.036.5451555
GGTATCA205750.036.114371
AACGCAG555200.035.420966
ACGCAGA569000.034.4678047
TCTCTGC15800.034.2603078
CGCAGAG573300.034.209288
GCAGAGT583850.033.6216939
GCTATTG34650.033.304698
GGGCTAT36100.032.626086
GGCTATT36300.032.1185847
CTATTGA36850.031.8006279
TTTAGGG38400.030.9821852
GAGTACT103100.028.84942812-13
ATTTAGG45700.027.207431
AGTACTT119650.026.94712612-13
ACTGTGC56850.026.3679858
Produced by FastQC (version 0.11.5)