FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437911
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC107100.31152638913572805No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT98290.285900362167607No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA93840.27295645524273315No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT66000.19197704652621897No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT60530.1760662216095763No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC57710.16786356598527422No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA57170.16629284469551422No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC56890.16547839661934238No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC54450.15838106338413063No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA48580.1413067412158139No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT47710.13877613469342284No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC47560.13833982322404506No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC46860.13630370303361547No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC45870.1334240473357222No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG39360.11448812956472697No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT38580.11221930992396255No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC38490.11195752304233589No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC34520.1004098128194709No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA34380.10000258878138496No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA304200.052.7348021
TATCAAC416000.038.605252
TCTCTGC18450.037.4098
ATCAACG436250.036.7041053
TCAACGC449250.035.508984
GCTATTG37450.033.9998058
CAACGCA483800.033.0170755
CTATTGA39200.032.4814839
AACGCAG501900.031.972626
ACGCAGA504100.031.7386597
CGCAGAG504250.031.729228
ACTGTGC60750.031.3413838
GCAGAGT509600.031.3256029
GGTATCA194600.031.3223921
GGGCTAT40900.030.8408996
CTGTGCA55450.030.6882869
TTTAGGG41600.030.4778292
GGCTATT41600.030.464977
CACTGTG57000.030.1673727
ATAGACA4450.026.7523174
Produced by FastQC (version 0.11.5)