FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10668078
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT661860.6204116617820005No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA422120.3956851459091319No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355500.33323715855845826No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC322650.3024443578309045No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT319400.29939788591721955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311620.2921051008438446No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC252540.23672492833291997No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC239850.22482962722994715No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC226870.21266248709467628No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT223760.2097472478172732No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT217250.20364493023016894No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA207230.19425242297628495No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC196300.1840069035865692No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC184330.1727865131844743No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT179600.16835272483009592No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC166330.15591374566252703No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA162050.15190177649619735No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC158650.14871469818649619No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT154260.14459961766308796No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA142350.13343546981939952No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG135000.1265457564146044No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT133220.12487722718187849No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA132330.12404296256551554No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA124160.11638460086249838No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT122600.11492229434392962No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG122170.11451922267534977No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC121220.11362871550058033No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC119840.11233513665723104No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG119490.1120070550665265No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC115550.10831379373116695No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG113040.10596098003782875No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC111130.10417059192855546No Hit
GTTCCGACCTGCACGAATGGCGTAATGATGGCCAGGCTGTCTCCACCCGA110220.10331757979272367No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT109430.10257705277370488No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA905650.065.6050641
GGTATCA474850.051.2424131
TATCAAC1329250.045.265892
ATCAACG1401200.042.9245533
TCAACGC1467550.040.9473954
CAACGCA1466500.040.915875
AACGCAG1505950.040.0806586
ACGCAGA1515300.039.5697757
CGCAGAG1522650.039.39838
TCTCTGC67750.039.33428
GCAGAGT1560150.038.474199
GCTATTG154700.034.5292938
CTATTGA158000.033.770469
GGGCTAT160550.033.456866
GGCTATT165100.032.5343677
TTTAGGG168500.032.2669682
AGAGTAC1605900.030.08843610-11
ACTGTGC196450.028.8564248
ATTTAGG189100.028.8181861
CACTGTG172700.028.6571257
Produced by FastQC (version 0.11.5)