FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10668078
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA433780.40661494975946No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC380710.3568684068489188No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT313430.29380175135577374No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC240920.22583261952152955No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC238310.22338606823084722No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT220150.2063633205531493No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC216880.20329810111999555No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT204390.19159027521171104No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA202580.1898936246997819No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT191440.17945125635564343No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC188830.17700470506496108No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC185220.1736207778008372No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA180440.1691401206477868No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC170350.15968199707576192No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC164110.15383277100148687No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA145330.13622885022025522No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG141420.13256371016409893No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT132060.12378987105268634No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA127990.11997475084077938No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT127080.1191217387049476No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT125900.11801563505628662No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC125340.11749070451115937No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA125020.11719074419965808No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG122830.11513789081782116No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC120340.11280382464395179No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG120310.11277570336474854No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC113800.1066733857776443No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT113530.1064202942648151No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG113070.105989101317032No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC110790.10385188409758533No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC107660.10091789730071339No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA783000.063.031581
GGTATCA433700.044.8819121
TATCAAC1125700.043.8532752
TCTCTGC64550.041.852948
ATCAACG1191750.041.1630743
TCAACGC1257700.038.967294
ACGCAGA1256400.038.756717
CGCAGAG1266600.038.538928
CAACGCA1268900.038.4656455
AACGCAG1282300.038.2851756
GCAGAGT1280300.038.0473449
GCTATTG151500.034.6042638
CTATTGA155600.033.730549
TTTAGGG160050.033.138672
GGGCTAT158750.033.1363686
GGCTATT167100.031.5870767
ACTGTGC200600.029.8127148
ATTTAGG185050.029.4658811
CACTGTG176600.029.3824547
CTGTGCA180000.029.0599
Produced by FastQC (version 0.11.5)