FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17190976
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATG208299212.116775685103628TruSeq Adapter, Index 23 (97% over 38bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTAT3877562.2555787408463606TruSeq Adapter, Index 23 (97% over 38bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA1662420.9670306095477069TruSeq Adapter, Index 23 (97% over 38bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTAT1549150.901141389529018TruSeq Adapter, Index 23 (97% over 38bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTAT1335940.7771170176725277TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC1257670.731587316508382TruSeq Adapter, Index 23 (97% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCT1176520.6843823177927768RNA PCR Primer, Index 29 (97% over 36bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA1148590.6681354217468514TruSeq Adapter, Index 23 (97% over 38bp)
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA1037040.6032467266547287TruSeq Adapter, Index 23 (97% over 38bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA1000800.5821658991321959TruSeq Adapter, Index 23 (97% over 38bp)
ACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC901690.5245135587415165TruSeq Adapter, Index 23 (97% over 38bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTAT834120.48520805334147404TruSeq Adapter, Index 23 (97% over 38bp)
CGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT698660.40641089836900474TruSeq Adapter, Index 23 (97% over 38bp)
ACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA687210.39975042720087567TruSeq Adapter, Index 23 (97% over 38bp)
CCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC640740.3727188031674292TruSeq Adapter, Index 23 (97% over 38bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA637380.3707642893573931TruSeq Adapter, Index 23 (97% over 38bp)
CACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT589790.3430811607206013TruSeq Adapter, Index 23 (97% over 38bp)
ACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG586350.34108011086746903TruSeq Adapter, Index 23 (97% over 38bp)
CCCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT571920.33268617209401025TruSeq Adapter, Index 23 (97% over 38bp)
ACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC566550.32956244020118464TruSeq Adapter, Index 23 (97% over 38bp)
GCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT552570.32143026667014135TruSeq Adapter, Index 23 (97% over 38bp)
ACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG543510.3161600597895082TruSeq Adapter, Index 23 (97% over 38bp)
CCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT542700.31568888235316017TruSeq Adapter, Index 23 (97% over 38bp)
CCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG529550.3080395202692389TruSeq Adapter, Index 23 (97% over 38bp)
CCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG522010.3036534982074316TruSeq Adapter, Index 23 (97% over 38bp)
AGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC508400.2957365538757078TruSeq Adapter, Index 23 (97% over 38bp)
CCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC506390.29456733579291833TruSeq Adapter, Index 23 (97% over 38bp)
ACCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT501180.29153667598628485TruSeq Adapter, Index 23 (97% over 38bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT500870.2913563488192875TruSeq Adapter, Index 23 (97% over 38bp)
GCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG497350.2893087629230592TruSeq Adapter, Index 23 (97% over 38bp)
CCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC476440.2771454046588163TruSeq Adapter, Index 23 (97% over 38bp)
CCCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT447160.2601132128856442TruSeq Adapter, Index 23 (97% over 38bp)
CAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG441260.2566811797073069TruSeq Adapter, Index 23 (97% over 38bp)
CCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC434010.25246385080172296TruSeq Adapter, Index 23 (97% over 38bp)
CGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG433310.2520566604246321TruSeq Adapter, Index 23 (97% over 38bp)
CAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC432020.25130626672970746TruSeq Adapter, Index 23 (97% over 38bp)
CAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT430510.2504278989162686TruSeq Adapter, Index 23 (97% over 38bp)
AGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC411890.23959663488565164TruSeq Adapter, Index 23 (97% over 38bp)
CCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC407970.2373163687739428TruSeq Adapter, Index 23 (97% over 38bp)
GTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA407740.2371825776500415TruSeq Adapter, Index 23 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATATCGTATG404610.23536185496390666TruSeq Adapter, Index 23 (97% over 38bp)
CCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT389950.22683412506654654TruSeq Adapter, Index 23 (97% over 38bp)
AGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT389430.22653164078642188TruSeq Adapter, Index 23 (97% over 38bp)
CGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC363840.21164592400105728TruSeq Adapter, Index 23 (97% over 38bp)
GCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT363680.2115528519148651TruSeq Adapter, Index 23 (97% over 38bp)
AGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT351260.20432813122419577TruSeq Adapter, Index 23 (97% over 38bp)
CACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC349000.20301348800673097TruSeq Adapter, Index 23 (97% over 38bp)
CCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT347710.2022630943118064TruSeq Adapter, Index 23 (97% over 38bp)
TGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC347400.202082767144809TruSeq Adapter, Index 23 (97% over 38bp)
CGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC343570.1998548540815833TruSeq Adapter, Index 23 (97% over 38bp)
CAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT322710.1877205808442755TruSeq Adapter, Index 23 (97% over 38bp)
CACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT318170.1850796603985719TruSeq Adapter, Index 23 (97% over 38bp)
TGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC315320.1834218138632734TruSeq Adapter, Index 23 (97% over 38bp)
CCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC310700.18073435737447366TruSeq Adapter, Index 23 (97% over 38bp)
GCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT308030.1791812169361414TruSeq Adapter, Index 23 (97% over 38bp)
CCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC307600.17893108570449984TruSeq Adapter, Index 23 (97% over 38bp)
ACCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT306110.178064351901835TruSeq Adapter, Index 23 (97% over 38bp)
GCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG303900.17677879371130528TruSeq Adapter, Index 23 (97% over 38bp)
GACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT302330.17586552386554433TruSeq Adapter, Index 23 (97% over 38bp)
CCACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT300370.1747253908096899TruSeq Adapter, Index 23 (97% over 38bp)
CTGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT299950.17448107658343542TruSeq Adapter, Index 23 (97% over 38bp)
CCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT289650.1684895610348127TruSeq Adapter, Index 23 (97% over 38bp)
CCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG289330.16830341686242828TruSeq Adapter, Index 23 (97% over 38bp)
CCGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT285110.1658486405891091TruSeq Adapter, Index 23 (97% over 38bp)
ACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT282110.16410353897300536TruSeq Adapter, Index 23 (97% over 38bp)
ACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT280600.1632251711595665TruSeq Adapter, Index 23 (97% over 38bp)
AACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC275510.1602643154175772TruSeq Adapter, Index 23 (97% over 38bp)
ACACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG268690.1562971177436348TruSeq Adapter, Index 23 (97% over 38bp)
AGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG264570.1539005115241857TruSeq Adapter, Index 23 (97% over 38bp)
GCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC262240.15254514926901186TruSeq Adapter, Index 23 (97% over 38bp)
ACCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC262040.15242880916127158TruSeq Adapter, Index 23 (97% over 38bp)
CCTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC259000.15066043952361982TruSeq Adapter, Index 23 (97% over 38bp)
CACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG255810.1488048148051629TruSeq Adapter, Index 23 (97% over 38bp)
ACCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT249650.14522153948676328TruSeq Adapter, Index 23 (97% over 38bp)
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT247610.14403487038781276TruSeq Adapter, Index 23 (97% over 38bp)
CACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC242080.14081806640879493TruSeq Adapter, Index 23 (97% over 38bp)
CACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT239440.13928237698662368TruSeq Adapter, Index 23 (97% over 38bp)
AGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT239160.13911950083578734TruSeq Adapter, Index 23 (97% over 38bp)
ATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA238030.13846217922705495TruSeq Adapter, Index 23 (97% over 38bp)
CTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT237830.1383458391193147TruSeq Adapter, Index 23 (97% over 38bp)
ACACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC237610.1382178650008004TruSeq Adapter, Index 23 (97% over 38bp)
GGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG235820.1371766210365252TruSeq Adapter, Index 23 (97% over 38bp)
ACCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC231720.13479164882785014TruSeq Adapter, Index 23 (97% over 38bp)
TGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT229740.1336398817612217TruSeq Adapter, Index 23 (97% over 38bp)
TGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTA229690.13361079673428664TruSeq Adapter, Index 23 (97% over 38bp)
CAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC228790.13308726624945552TruSeq Adapter, Index 23 (97% over 38bp)
TGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG225410.13112111842864535TruSeq Adapter, Index 23 (97% over 38bp)
CATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT220980.1285441850421989TruSeq Adapter, Index 23 (97% over 38bp)
CCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC220570.12830568782133137TruSeq Adapter, Index 23 (97% over 38bp)
TGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG219200.1275087580833107TruSeq Adapter, Index 23 (97% over 38bp)
GGCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC217460.12649659914597053TruSeq Adapter, Index 23 (97% over 38bp)
CCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG212460.12358809645246437TruSeq Adapter, Index 23 (97% over 38bp)
ACCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC211730.12316345505921245TruSeq Adapter, Index 23 (97% over 38bp)
CTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG211510.12303548094069819TruSeq Adapter, Index 23 (97% over 38bp)
GGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG211450.12300057890837612TruSeq Adapter, Index 23 (97% over 38bp)
ACGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT209800.12204077301951907TruSeq Adapter, Index 23 (97% over 38bp)
TGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT207180.12051671760812184TruSeq Adapter, Index 23 (97% over 38bp)
CCCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT206140.11991174904787255TruSeq Adapter, Index 23 (97% over 38bp)
CCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT204880.119178806369109TruSeq Adapter, Index 23 (97% over 38bp)
CCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG204360.11887632208898435TruSeq Adapter, Index 23 (97% over 38bp)
CCCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT202990.11807939235096367TruSeq Adapter, Index 23 (97% over 38bp)
AACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC201550.1172417435752339TruSeq Adapter, Index 23 (97% over 38bp)
ACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT201370.11713703747826766TruSeq Adapter, Index 23 (97% over 38bp)
CGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG201150.11700906335975339TruSeq Adapter, Index 23 (97% over 38bp)
CGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT199260.11590964934160806TruSeq Adapter, Index 23 (97% over 38bp)
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGT199080.11580494324464186TruSeq Adapter, Index 23 (97% over 38bp)
ACTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC198680.11557226302916135TruSeq Adapter, Index 23 (97% over 38bp)
CCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT196880.11452520205949912TruSeq Adapter, Index 23 (97% over 38bp)
CAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG194530.11315820579355122TruSeq Adapter, Index 23 (97% over 38bp)
ACCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT193840.11275683242184736TruSeq Adapter, Index 23 (97% over 38bp)
AAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC192060.11172140546295917TruSeq Adapter, Index 23 (97% over 38bp)
AGCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT191390.11133166610202934TruSeq Adapter, Index 23 (97% over 38bp)
AACCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT190630.11088957369261641TruSeq Adapter, Index 23 (97% over 38bp)
ACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG187110.10884198779638807TruSeq Adapter, Index 23 (97% over 38bp)
GCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG186970.10876054972096989TruSeq Adapter, Index 23 (97% over 38bp)
ACTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT180320.10489224113860668TruSeq Adapter, Index 23 (97% over 38bp)
CGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG180210.10482825407934955TruSeq Adapter, Index 23 (97% over 38bp)
GGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC178210.10366485300194707TruSeq Adapter, Index 23 (97% over 38bp)
ATGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT177070.10300171438782765TruSeq Adapter, Index 23 (97% over 38bp)
AACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCG176010.10238511181680436TruSeq Adapter, Index 23 (97% over 38bp)
AGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTC174360.10142530592794731TruSeq Adapter, Index 23 (97% over 38bp)
AACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCT174200.10133223384175512TruSeq Adapter, Index 23 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGTGG77950.071.4120947
CCCGCCA92350.068.665931
CCCGCAA27300.067.3356861
CCCGGCC16650.067.327851
CGGTGTG31650.067.230636
GTGTGGA97150.067.125588
CCCCCCA77400.066.770541
GCCCCAA51100.066.556281
GGCCCAA24950.066.062631
CCTGCCA66000.065.420951
ACCCCCA88400.064.963741
CCCGCCC16200.064.2134551
CCCGGTG5350.063.921274
TAGCCCC11800.063.6021921
GCCCGCA17900.063.418222
CCGGTGT14750.063.1141745
GGGTGTG48950.062.6819766
TGTGGAA103500.062.5483479
CCGGCCC12300.062.1752781
CCGCCCA66000.061.102741
Produced by FastQC (version 0.11.5)