FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17190976
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC183941010.699857878924385Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC3278621.9071750201966429Illumina Single End PCR Primer 1 (100% over 49bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG1421780.8270501919146417Illumina Single End PCR Primer 1 (100% over 48bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC1332490.7751101508140085Illumina Single End PCR Primer 1 (100% over 49bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC1139690.6629582869524104Illumina Single End PCR Primer 1 (100% over 49bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG1000700.5821077290783258Illumina Single End PCR Primer 1 (100% over 48bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG902420.5249382001347683Illumina Single End PCR Primer 1 (100% over 48bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG853860.4966908219754364Illumina Single End PCR Primer 1 (100% over 48bp)
GGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG762260.44340705263040336Illumina Single End PCR Primer 1 (100% over 45bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC720360.4190338000588215Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCC638560.3714506959930605Illumina Single End PCR Primer 1 (98% over 50bp)
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG596880.34720541753999307Illumina Single End PCR Primer 1 (100% over 48bp)
GCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC595050.3461409055541698Illumina Single End PCR Primer 1 (100% over 47bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG555530.323152100264697Illumina Single End PCR Primer 1 (100% over 48bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG551780.3209707232445674Illumina Single End PCR Primer 1 (100% over 45bp)
GTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC505990.2943346555774378Illumina Single End PCR Primer 1 (100% over 47bp)
GGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT492770.2866445744558075Illumina Single End PCR Primer 1 (100% over 46bp)
GGCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG480070.27925697761430185Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG478440.27830880573621886Illumina Single End PCR Primer 1 (100% over 44bp)
GGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC473740.27557481320432303Illumina Single End PCR Primer 1 (100% over 47bp)
TGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC461040.26818721636281734Illumina Single End PCR Primer 1 (100% over 47bp)
GCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT459070.26704126630157593Illumina Single End PCR Primer 1 (100% over 46bp)
GGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG451040.262370210975805Illumina Single End PCR Primer 1 (100% over 45bp)
GTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT447470.26029354005264155Illumina Single End PCR Primer 1 (100% over 46bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT446390.2596653034708442Illumina Single End PCR Primer 1 (100% over 46bp)
GTGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG429940.2500963296092089Illumina Single End PCR Primer 1 (100% over 45bp)
GGCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG429490.24983456436679335Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT429090.24960188415131287Illumina Single End PCR Primer 1 (100% over 46bp)
GGGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG424120.24671083247396774Illumina Single End PCR Primer 1 (100% over 44bp)
GCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG412160.23975369403110097Illumina Single End PCR Primer 1 (100% over 45bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG385460.224222289647778Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG380980.22161627123439645Illumina Single End PCR Primer 1 (100% over 44bp)
GCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT379430.22071463539940955Illumina Single End PCR Primer 1 (100% over 46bp)
GGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG366770.21335030657945192Illumina Single End PCR Primer 1 (100% over 45bp)
GGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG365920.21285586112155586Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT363260.21130853768861058Illumina Single End PCR Primer 1 (100% over 46bp)
GGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG362510.21087226228458464Illumina Single End PCR Primer 1 (100% over 45bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC359670.20922023275467316Illumina Single End PCR Primer 1 (98% over 50bp)
ACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG356800.20755075220860061Illumina Single End PCR Primer 1 (100% over 48bp)
GGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG347930.20239106843032065Illumina Single End PCR Primer 1 (100% over 45bp)
GGGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG341230.1984936748210224Illumina Single End PCR Primer 1 (100% over 44bp)
GGCAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG330010.19196699477679452Illumina Single End PCR Primer 1 (100% over 44bp)
GCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC308570.17949533522704006Illumina Single End PCR Primer 1 (100% over 47bp)
GGGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG308040.1791870339415284Illumina Single End PCR Primer 1 (100% over 45bp)
GGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG307580.17891945169372583Illumina Single End PCR Primer 1 (100% over 45bp)
AGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC304700.17724415414226627Illumina Single End PCR Primer 1 (100% over 47bp)
GGGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG304000.1768369637651754Illumina Single End PCR Primer 1 (100% over 44bp)
GGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG299510.17422512834640685Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG298550.17366669582925368Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG282700.1644467422908391Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG280850.1633705962942418Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG271410.15787934320890218Illumina Single End PCR Primer 1 (100% over 44bp)
TGGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG268970.15645999389447116Illumina Single End PCR Primer 1 (100% over 44bp)
TGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG267150.1554012989140349Illumina Single End PCR Primer 1 (100% over 45bp)
GGCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG262790.15286508456529752Illumina Single End PCR Primer 1 (100% over 44bp)
GCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG261030.15184129161718335Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG260840.1517307685148301Illumina Single End PCR Primer 1 (100% over 44bp)
GTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC260620.15160279439631583Illumina Single End PCR Primer 1 (100% over 47bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCC258970.1506429885074588Illumina Single End PCR Primer 1 (98% over 50bp)
AGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT258780.15053246540510556Illumina Single End PCR Primer 1 (100% over 46bp)
GGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG256060.1489502399398382Illumina Single End PCR Primer 1 (100% over 44bp)
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC249540.14515755242750616Illumina Single End PCR Primer 1 (100% over 47bp)
GGCCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG247090.14373238610768813Illumina Single End PCR Primer 1 (100% over 44bp)
GCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT244220.14206290556161558Illumina Single End PCR Primer 1 (100% over 46bp)
GGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC240950.14016074480006252Illumina Single End PCR Primer 1 (100% over 47bp)
GGGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG239470.1392998280027847Illumina Single End PCR Primer 1 (100% over 45bp)
AGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC237270.138020086817642Illumina Single End PCR Primer 1 (100% over 47bp)
GGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT227990.13262190581849453Illumina Single End PCR Primer 1 (100% over 46bp)
TGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG226170.13156321083805828Illumina Single End PCR Primer 1 (100% over 45bp)
GCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG225840.1313712496602869Illumina Single End PCR Primer 1 (100% over 45bp)
GTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT225370.13109785040709732Illumina Single End PCR Primer 1 (100% over 46bp)
GGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT218990.12738660097018342Illumina Single End PCR Primer 1 (100% over 46bp)
GCAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG217170.12632790598974716Illumina Single End PCR Primer 1 (100% over 45bp)
GCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC214730.12490855667531615Illumina Single End PCR Primer 1 (100% over 47bp)
GGGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG214080.12453045132516036Illumina Single End PCR Primer 1 (100% over 44bp)
GCGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG209490.1218604458525217Illumina Single End PCR Primer 1 (100% over 44bp)
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG208480.12127292830843345Illumina Single End PCR Primer 1 (100% over 48bp)
GCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG207400.12064469172663611Illumina Single End PCR Primer 1 (100% over 45bp)
GGGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG206890.12034802445189847Illumina Single End PCR Primer 1 (100% over 44bp)
GGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG202630.11786998015703122Illumina Single End PCR Primer 1 (100% over 45bp)
GGGGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG201840.11741043673145725Illumina Single End PCR Primer 1 (100% over 44bp)
GCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG201150.11700906335975339Illumina Single End PCR Primer 1 (100% over 45bp)
GGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT200960.11689854025740017Illumina Single End PCR Primer 1 (100% over 46bp)
GGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT199550.11607834249783142Illumina Single End PCR Primer 1 (100% over 46bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCC199000.11575840720154573Illumina Single End PCR Primer 1 (98% over 50bp)
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC198760.11561879907225744Illumina Single End PCR Primer 1 (100% over 47bp)
CAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG198490.11546173992680812Illumina Single End PCR Primer 1 (100% over 48bp)
TGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG198220.11530468078135878Illumina Single End PCR Primer 1 (100% over 45bp)
ATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC189330.1101333629923048Illumina Single End PCR Primer 1 (100% over 47bp)
AGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG189070.10998212085224247Illumina Single End PCR Primer 1 (100% over 45bp)
GGCCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG186600.10854532052165043Illumina Single End PCR Primer 1 (100% over 44bp)
AGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT185710.10802760704220633Illumina Single End PCR Primer 1 (100% over 46bp)
AGGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG183760.10689329099173892Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG182110.10593348510288189Illumina Single End PCR Primer 1 (100% over 44bp)
ATGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT179570.10445596573458074Illumina Single End PCR Primer 1 (100% over 46bp)
GGTGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG179510.10442106370225866Illumina Single End PCR Primer 1 (100% over 44bp)
GTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG178980.10411276241674702Illumina Single End PCR Primer 1 (100% over 45bp)
GCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT178970.10410694541136Illumina Single End PCR Primer 1 (100% over 46bp)
AGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT178850.10403714134671585Illumina Single End PCR Primer 1 (100% over 46bp)
GCGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG175030.10181504528887714Illumina Single End PCR Primer 1 (100% over 45bp)
GTGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG174700.10162308411110572Illumina Single End PCR Primer 1 (100% over 44bp)
TGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT174310.10139622090101226Illumina Single End PCR Primer 1 (100% over 46bp)
GCCAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG173920.10116935769091877Illumina Single End PCR Primer 1 (100% over 44bp)
TGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC173600.10098321351853438Illumina Single End PCR Primer 1 (100% over 47bp)
GGGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG171970.10003504164045136Illumina Single End PCR Primer 1 (100% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAC91600.066.946762
GGGGCTA77550.065.120241
GGCGTTA32700.065.025911
ACGCTGA91700.064.8024141
GCTGACT96900.063.534233
GGGCCTA47750.063.019081
GGGCCGA38200.062.8699841
CTGACTG99100.062.1237834
CTGTAAC100600.061.7437328
GCGGCTA37550.061.430281
GGCGGTA63400.060.1149441
TGACTGT103100.059.8895075
TGTAACT105200.058.7806329
GGCCCGA22000.057.602951
GGCCTGA84200.057.4405021
GACTGTA108700.057.1870886
GGCGTGA66100.056.4387321
GGCCATA38250.056.33531
TGCGGCA21200.054.1795851
GCCCGAG33000.054.079962
Produced by FastQC (version 0.11.5)