FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12757125
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG200190.15692407184220583No Hit
GGAGTCTTGGAAGCTTGACTNCCCTACGTTCTCCTACAAATGGACCTTGA129760.10171570788872886No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA84800.046.3633814-15
TGTCGGG32900.039.3632355
CGGGGAT142950.038.538668
TCGGGGA150250.037.0179447
TGGTCGT149050.037.0116614-15
CTGTCGG35150.036.0916334
GGTGTGC91350.036.0592356
GTGCTCT120950.034.0065659
CCCCGTT30400.033.185422
GTCGGGG43050.032.9386836
GTCCCGT19800.032.9279631
TGTGCTC134150.031.0216278
CGGTGTG37250.030.6275945
TCCGACC24650.029.8346759
TCTGTCG41900.029.3310643
ATCGGGG109500.029.197526
CTATCGG82300.028.4206544
CCCCCGT23350.028.2991091
TATCGGG88950.028.2767525
GCCCCGT23050.028.0940781
Produced by FastQC (version 0.11.5)