FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16867069
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC621450.3684398279274247No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC360380.21365893505267572No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC213330.12647722019753402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA63000.059.110514
GCGACAT62900.058.9020735
CTGCGAC68750.054.377863
GCTGCGA79850.046.640432
CGACATC84150.044.1959726
GGCTGCG97900.038.576591
CTAGGCG208350.034.1037037
TAGGCGG214350.033.1712468
TCGACCG13950.032.009123
GCTAGGC229650.030.9414146
TGGCTAG242550.029.9234224
GGCTAGG244200.029.6238085
ACCCGAC34650.028.241263
GGCTGGC270500.026.3780141
AGGCGGG275650.025.8806639
CTGGCTA287250.025.5333253
GCTGGCT289300.025.0240672
GCTCGCG17750.024.8855959
CCGACTC39400.024.35245
GACATCT154900.024.3156157
Produced by FastQC (version 0.11.5)