FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16867069
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG244540.14498073138848247No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC51500.042.2467969
TTCCGAC52450.041.391038
TGTCGGG34600.039.274715
CGGGGAT170300.037.9925088
TCGGGGA173200.037.5755737
CCCCGTT47900.033.337362
GTCCCGT26700.033.1077081
CCGTTAA22750.032.7927174
GTTCCGT24850.032.7037241
CCCCCGT36350.032.4246061
CTGTCGG42800.031.863784
TTTCCGA70700.031.4455627
CCCGTTA23300.031.2085273
CCGTTTA27700.031.0497424
GCCCCGT35600.030.7047271
CCGTTTC79250.030.6993964
CCCGTTT57950.030.508953
ATCGGGG129200.030.2963226
TCTATCG99750.030.2550963
GCTCCGT49500.030.2435511
Produced by FastQC (version 0.11.5)