FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848496
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160370.5629988597491449No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC99550.34948267436570035No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT91850.3224508652987401No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA85020.29847329959389096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77140.2708095781071836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73930.2595404732883599No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT65490.22991080205132813No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC64410.2261193275328454No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT55430.19459391903657228No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC49960.17539080272536806No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC49860.17503974026995298No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC49480.17370570293937573No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA46340.1626823418393426No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT45990.16145362324538987No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA45520.15980362970493903No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT45370.15927703602181642No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC45020.15804831742786368No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC39050.13708988883958412No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC38750.1360367014733389No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT36210.1271197151057962No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC36110.12676865265038112No Hit
ATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATC35050.1230473906229814No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG33890.11897506614016659No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG32980.11578039779588949No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC31930.11209424201403127No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT30880.10840808623217306No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA30830.1082325550044655No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG30570.10731979262038632No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT30250.10619639276305813No Hit
GGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCA29430.10331768062865455No Hit
ACATATCAGCGTGCCTTCTCCCGAAGTTACGGCACCATTTTGCCTAGTTC29370.10310704315540552No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA220400.068.9943161
GGTATCA109400.054.48971
TATCAAC323250.047.8091772
ATCAACG340800.045.277353
TCAACGC351700.043.721884
CAACGCA359850.042.7977755
AACGCAG365850.042.4358446
ACGCAGA372550.041.3373767
CGCAGAG374700.041.0835888
GCAGAGT390200.039.5278369
TCTCTGC13700.038.207618
AAGTACG1951.8189894E-1236.6109241
CTATTGA31650.034.768499
GCTATTG31750.034.658988
GGGCTAT35250.031.5557066
TTTAGGG35150.031.477362
GGCTATT35650.031.2016457
AGAGTAC394150.030.25856210-11
TAGTACT7750.029.1670534
GAGTACT85500.028.80296712-13
Produced by FastQC (version 0.11.5)