FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25811606
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG3497971.3551926989742522TruSeq Adapter, Index 22 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTAT425920.1650110419320673TruSeq Adapter, Index 22 (97% over 37bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTAT326920.1266562026400062TruSeq Adapter, Index 22 (97% over 37bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTA325050.12593172234226727TruSeq Adapter, Index 22 (97% over 37bp)
ACAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCT275260.10664195013669432TruSeq Adapter, Index 22 (97% over 37bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTAT272680.10564239977938607TruSeq Adapter, Index 22 (97% over 37bp)
ACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTC265020.10267474251699023TruSeq Adapter, Index 22 (97% over 37bp)
CCAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCT259410.10050130162377344TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAGA188700.032.1665533
AACGCCA63500.031.6930261
GCCAGAT852150.031.4580524
TTGAAAA4783700.031.43318668-69
CCGACGC19750.031.1358321
ACGGCCA60600.030.797021
TGAAAAA3103400.029.58605268-69
ACGCCAG144950.029.4194952
CCAGATC1742650.027.9412945
TCGGTCG16900.027.4675568-69
CTACTAA195850.025.8211351
TAGCCGC14250.024.7451761
TACGCCA33700.024.4998191
CGCGGCA16250.024.3459111
AACGCAG72400.023.7581711
CAGATCG4168150.023.6485796
CGCAGAT265650.023.3506134
CACGCCA84000.023.3440991
CCGCTAG15100.023.0594982
CCCAGAT689550.022.8106884
Produced by FastQC (version 0.11.5)