FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25811606
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3255951.2614286766968317Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC392140.15192390585847312Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC301540.11682341656695054Illumina Single End PCR Primer 1 (100% over 49bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG300280.11633526406686977Illumina Single End PCR Primer 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTA40400.059.459911
GGCCGTA46350.049.4712071
GCGTTAG67350.044.4629062
CGTTAGA98300.043.0209473
GCGGCTA23100.041.9250221
CGGTGTG161300.040.905655
CCCGAGA72550.039.121863
GGTGTGC448350.039.0865176
CCGGTGT84650.038.8034444
GTGTGCT702150.038.4078647
GGCGTAG80600.038.2265171
CCGTAGA89100.038.034373
GTGCTCT707600.037.9779179
TGTGCTC706900.037.9349678
CGCGAGA24700.036.8943633
GCCGTTA29900.036.3596271
GCCGTAG78050.036.2392652
GGCCCGA34300.034.325171
GGCGTAA27150.034.097291
CCGAGAT145350.034.028584
Produced by FastQC (version 0.11.5)