FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37526083
Sequences flagged as poor quality0
Sequence length41
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN841010.2241134519688612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGT26550.020.0044421
TCCGACC23400.018.6106579
CCCTACG26850.018.5662722
TACGCCG13750.017.68401735
GGTCCGT22500.017.50591
CGACCTG25150.017.24766211
GGCCCGT28150.016.2715681
GCGCGCT14500.016.04602831
GCCCCGT30750.015.9390431
CGCGCAG42900.015.86211535
GTTCCGT24900.015.675431
CCGACCT27900.015.54733210
CCCGTTA29400.015.5257583
CCCCCGA34200.015.4255371
TCGACCG45300.015.21382426
AGCGCGC44500.015.13548133
CGATCGG7050.015.1254247
TCGATCG6950.015.0928636
CCCCGTT49650.015.0411692
TCCCCGT41000.015.0406611
Produced by FastQC (version 0.11.5)