FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10209155
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG553440.5421016724694648TruSeq Adapter, Index 10 (97% over 36bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTAT115800.11342760492910531TruSeq Adapter, Index 10 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG8500.039.1139763
GCTCGCG9500.035.9920439
GCGCGGT26600.031.600316
CCGCTCG11300.029.4184157
CGCGGTG29250.028.8997337
GGGCGCG30200.027.9940644
CGACCGC13050.026.204444
CGCGTCG6750.025.3279812-13
CTCGCGT6750.025.32773210-11
CGTCGCA7000.025.10183714-15
CTCGACC14150.023.4960292
TCGCGTC7300.023.41947710-11
GGCGCGG37400.023.23985
CGGGCGC36600.022.9691583
TCCGATC44550.022.91883716-17
CCGGGCG36900.022.911132
CGCTCGC14750.022.5373888
CGTGTAG20800.022.03637958-59
AATGCGG20950.021.9907764
GCGCGTG27400.021.05850614-15
Produced by FastQC (version 0.11.5)