FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10209155
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC541000.5299165308000515Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC112480.1101756217826059Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTG57250.038.5128145
GGTGTGC161800.038.283616
CCCCGTT25350.037.3050542
CCGGTGT31050.037.1916774
TCCGACC22600.036.7808889
GTGTGCT255050.036.765097
TGTGCTC257650.035.785158
GTGCTCT261850.035.6090439
CCCCCGT20650.034.747871
CCCGTTT30900.033.373053
CCGTTTA16400.032.7442974
TTCCGAC25450.032.4764758
GTCCCGT14900.031.2543951
CCGTTAT10700.030.201344
GTTCCGT14050.029.7630631
GCCCCGT18350.029.0036871
CCCGTTA14850.028.8012123
CCCCGAA21600.027.9410382
TCCCCGT26900.027.7343921
CGTCCGT5250.027.1539631
Produced by FastQC (version 0.11.5)