FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18693671
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC608240.3253721540300993No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC580210.31037777438150055No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC254890.13635096070750363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA43700.053.4917564
GCGACAT44600.052.212885
CTGCGAC52800.044.2725333
GCTGCGA59750.039.422062
CGACATC69800.033.4265986
GGCTGCG76300.031.0628341
TGGCTAG255300.024.8951874
ACCCGAC39000.024.6399883
CTAGGCG256050.024.1421347
GGCTGGC265350.023.64441
TAGGCGG264950.023.3479148
GCGCGGT62800.023.2025156
ACTCGGG169900.022.7410261
GCTAGGC277450.022.5055076
GCTGGCT282500.022.4507352
GGCTAGG290650.021.9753935
CTGGCTA300650.021.467063
CCCGACT44550.021.3697054
CTCGGGA184000.021.3033752
GGACGAC158950.021.24207578-79
Produced by FastQC (version 0.11.5)