FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682543
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC211330.21825877767854993No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC173640.17933305331047847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA31200.056.4817164
GCGACAT32900.053.84895
CTGCGAC34750.050.5778273
GCTGCGA41250.043.071062
CGACATC49350.035.9958846
GGCTGCG53650.033.740851
ACCCGAC19650.028.5254613
CTAGGCG97050.027.5537837
TAGGCGG99200.026.8129588
GCTAGGC109800.024.613786
CCCGACT24150.023.995584
TGGCTAG116100.023.8110964
GGCTAGG116600.023.5854365
CCGACTC24650.022.7367825
TTGATCG41550.021.09126922-23
TCGATCG13500.020.75816216-17
CGACTCC27100.020.3309236
GCGCGGT25600.020.2234346
AACCCGA31200.019.6414262
AGGCGGG137400.019.4621169
Produced by FastQC (version 0.11.5)