FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682543
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG98840.1020806207625414No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC30050.040.9427349
TTCCGAC30900.039.9947978
CGGGGAT81250.036.446298
CCCCGTT26450.034.3414462
TCGGGGA88150.033.863397
TGTCGGG19250.032.8486335
GCCCCGT23000.030.7896251
GTCCCGT14800.030.507591
CCGTTTC44250.029.6572154
CCCCCGT22300.029.6248341
ATCGGGG60500.029.6197476
ATAGCGC52750.029.12747294-95
CCCGTTA13400.029.1018073
CGATCGG53050.029.09787486-87
CCGTTTA14400.029.0572664
CCCGTTT32550.028.9284463
TTTCCGA44050.028.7031027
TCCGTTT49000.028.6310213
GTTTCCG38100.028.569716
CTATCGG47800.028.4493714
Produced by FastQC (version 0.11.5)