FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18286977
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1186090.6485981800053666Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC189300.10351628921499709Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC56500.046.1098828
TCCGACC57100.045.5423859
TTTCCGA73750.035.775277
GTTTCCG64200.034.7397776
GGCGTTA20200.034.3156851
GGCCGTA23150.034.04461
CGGTGTG53450.031.8820635
GTGCTCT216050.031.1892819
CGTTTCC72600.030.860655
GCTCCGT56900.030.6228051
GTCCCGT23150.030.5581071
CCGGTGT32900.030.4419964
CTCCGTT60400.030.3291152
TCCGTTT81200.030.278243
GTTCCGT25500.030.1624071
GTGTGCT229700.029.6663157
GGTGTGC163550.029.5366636
CCGTTTC82250.028.9703944
GCGTTAG27950.028.8653262
CCCCGTT42500.028.6981262
Produced by FastQC (version 0.11.5)