FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20402200
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA715290.3505945437256766No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATG534230.2618492123398457TruSeq Adapter, Index 3 (97% over 36bp)
TCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCA243280.11924204252482576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGG118900.051.1521768-69
TCGGTCG24250.048.388368-69
TAGTGCG134650.045.740247
AGTGCGC138150.044.705868
GTGCGCT145000.042.9495859
GTAGTGC166250.037.459796
ATACCGG44150.036.4082269
CTAGGCG35200.034.4322247
TAGGCGG35100.034.0405278
AATACCG49950.032.352738
GGCTGTA209350.030.2466132
GCTGTAG215700.029.2764643
GGGCTGT214100.029.0226151
CTACTAA208050.028.915811
GCTCACT171600.027.5313433
TGCAACC127300.026.9759659
TGTAGTG233250.026.9630895
GGAGCGG124500.025.02984266-67
GGGAGCG122750.024.96649266-67
CCGATCG128400.024.5357718-19
Produced by FastQC (version 0.11.5)