FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20402200
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC493620.2419444961817843Illumina Single End PCR Primer 1 (100% over 50bp)
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG439370.21535422650498476No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC106150.069.517079
TTCCGAC112850.065.347578
GTTTCCG111550.058.0679746
TTTCCGA127750.057.87447
GCTCCGT92350.052.695251
CGTTTCC121650.051.5460435
CTCCGTT98950.051.3202172
CCGTTTC127050.050.2882424
TCCGTTT124900.049.8203243
GTTCCGT30550.040.2506681
GTCCCGT24400.036.9699021
CCCCGTT51700.036.443532
CCCGTTA26550.034.6819463
CGACCTG111750.034.0132810-11
CCCCCGT39550.033.492091
CCGACCT117100.032.60112810-11
CCGTTAT22700.032.2026024
GCCGGTT119500.031.92797318-19
TGGGCCG123400.030.99414316-17
CCCGTTT69700.030.7802353
Produced by FastQC (version 0.11.5)