FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5105136
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327390.6412953543255263No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA174860.3425178095157504No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC157990.3094726565560643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156610.3067694964443651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154360.3023621701752901No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT136450.26727985307345387No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC105510.20667421984448603No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC102050.19989673144848638No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA97550.19108207891033657No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT96250.18853562373264884No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC92960.18209113332142376No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT88740.17382494805231438No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC85060.16661652108778296No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT82250.1611122602806272No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC77740.15227801962572593No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC68810.13478583136668643No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC67260.13174967327021259No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT65950.12918362997577343No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA60970.11942874783355428No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA60150.11782252225993589No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT58530.11464924734620194No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC55230.1081851688182254No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG54480.10671606006186711No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54050.10587377104155501No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG53570.1049335414374857No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC52250.10234791002629508No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT51980.10181903087400611No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA51850.10156438535623732No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA466350.055.5048981
AAGTACG9450.047.2153971
TATCAAC647400.041.265422
GGTATCA272550.040.512211
ATCAACG677900.039.443913
TCAACGC707200.037.8349464
CAACGCA741600.036.200255
GCTATTG62450.034.1948668
CTATTGA63700.033.7106139
AACGCAG803550.033.6970986
TCTCTGC31450.033.2882428
ACGCAGA823900.032.6406567
CGCAGAG826400.032.5851028
GGGCTAT66450.031.9577646
ACTGTGC93200.031.9119248
ATACGGG19550.031.6475981
GCAGAGT852350.031.5930429
TTTAGGG67550.031.3502182
CACTGTG81700.030.943247
GGCTATT69200.030.7734247
Produced by FastQC (version 0.11.5)