FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5105136
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA169060.33115670179991286No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC161890.3171120220891275No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT150030.29388051562191486No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC102740.201248311504336No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC100920.19768327425557322No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT97740.191454253128614No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT96280.18859438808290319No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA90970.1781930980878864No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT90300.17688069426553965No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC86970.17035785138730877No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC84600.16571546771721654No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC79040.15482447480341366No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC71520.14009421100632774No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC70050.13721475784386547No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA67460.13214143560524147No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT61440.12034938932087215No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA59980.11748952427516134No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG58490.11457089487919617No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT58070.1137481939756355No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT56350.11037903789438713No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC53910.10559953740703479No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA52250.10234791002629508No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT51350.10058497951866512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA427500.052.1017151
TATCAAC568950.039.2111852
TCTCTGC33500.038.0177428
ATCAACG591900.037.3085983
TCAACGC617200.035.8364334
GCTATTG66600.034.1355138
CTATTGA67900.034.095519
CAACGCA660000.033.644185
TTTAGGG70400.032.729452
AACGCAG704050.031.6980346
ACGCAGA702150.031.6309367
CGCAGAG704700.031.5346038
TTCTCTG40700.031.4372357
GGTATCA265950.031.38691
GGGCTAT72700.031.3522646
GCAGAGT723500.030.62479
GGCTATT75800.030.1482667
ACTGTGC90800.029.4947018
ATTTAGG78800.029.391641
CACTGTG79650.028.9898687
Produced by FastQC (version 0.11.5)