FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10042318
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC443220.44135228539865No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG416080.4143266524720687No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC383940.3823220893821526No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG170410.16969189782677666No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATG106640.1061906225236046TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA58600.069.218124
GCGACAT61100.066.6191565
CTGCGAC69750.058.368763
GCTGCGA73100.055.763292
CGACATC73550.055.4069376
TCGACCG17100.044.171783
GGCTGCG98150.041.9279671
CGCACTA109800.039.5802237
GTCCGCA113800.038.1890034
TCCGCAC119250.036.403855
ACATCTG113150.036.393528
GACATCT116500.035.2654727
CCGCACT127450.034.47166
TGTCCGC125850.034.4259153
GTGTCCG126600.034.2246972
GCACTAA128900.033.973288
CACTAAG130800.033.4434749
CTCGACC23000.033.2564962
GCGCGGT50450.032.6683966
CATCTGT126950.032.5870449
Produced by FastQC (version 0.11.5)