FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10042318
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG133740.13317642400887922No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG132400.1318420707251055No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130940.13038822311741172No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA101630.10120173449994314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC52250.050.6225749
TTCCGAC54250.048.742198
TTTCCGA65100.040.9042247
CGTTTCC62900.036.7763865
GTTTCCG64150.036.6498686
CTCCGTT51700.036.251412
CGGGGAT108100.035.928868
TCACCCC185650.035.1232649
TCGCGCG35050.035.0956427
TCGGGGA112350.034.7762077
CCGTTTC65050.034.4734574
TCCGTTT62700.034.4022373
TTCACCC191650.034.136788
GCTCCGT53200.032.3908041
TGTCGGG27500.031.5226945
CCGATCG71400.031.27270786-87
CGCGCGT39650.030.7892078
ATCGGGG78300.030.7487666
ACCCGAT72750.030.55706684-85
ATAGCGC64200.030.5413994-95
Produced by FastQC (version 0.11.5)