FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11859788
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1190821.0040820291222743No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA588210.49597007973498347No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG552700.4660285664465503No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA537920.4535662863450847No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG482210.40659242812772034No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG364950.3077205090006668No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA359930.3034877183302096No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG261070.2201304104255489No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG246110.20751635695342951No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA230960.19474209825673106No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG207810.1752223564198618No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG207640.17507901490313316No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA195500.16484274423792397No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA191530.16149529822961423No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC187660.1582321707605566No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA185000.1559892976164498No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182880.15420174458430452No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG172970.14584577734441795No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG172100.1451122060529244No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT146700.12369529708288209No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC132700.11189070158758319No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA127980.10791086653488241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTA66650.062.687125
GGTGTTA99150.061.66392
GTGCTAG63850.061.260633
TGCTAGT69950.058.304884
GATAGTA110450.056.7419175
AATACCG42600.056.381898
ATACCGG43150.055.5532239
CGACCTA8000.052.8279381
GGTGCTA73200.052.7214432
GTGATAG103200.051.6553423
GCCCCGT29400.051.0391431
GTAGCAA1048500.050.2700969
ATAGTAG143550.050.1945156
AGTAGCA1056000.049.872618
CGCGATA4350.049.1064383
TAGTAGC1067200.049.1045727
GGTGATA103550.048.9127582
GTGTTAG135800.047.189913
CGCGAAT2550.046.5474175
TGATAGT118500.046.1565784
Produced by FastQC (version 0.11.5)