FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14414087
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC395010.27404441224754644No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT83400.051.0652738
TGCGACG18500.049.0185936
GTCGACG10250.047.247146
CCGACGC49250.045.888097
GACGCTC97250.044.2285969
TCGACGC19100.040.2699287
CTAGGCG103200.039.979687
TAGGCGG105550.038.5947538
GCCGACG24200.037.080516
GTGCGAC19550.035.9468575
GCGACGC42200.034.2026987
GCTAGGC123850.033.390386
CCCGACG29950.032.022396
GCGACAT23450.031.7908955
GCGCGGT44350.030.7246886
TGCGACA24300.030.4879024
GGCTAGG143650.029.584445
TCCGACG12400.029.4827696
TGGCTAG148550.028.9324464
TGTCGAC15100.028.3016345
Produced by FastQC (version 0.11.5)