FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14414087
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN448960.31147307491622606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC47550.048.369989
TTCCGAC49850.046.5153548
TTTCCGA65400.035.816287
CTCCGTT57350.035.7638852
ATAGCGC90250.034.8739894-95
GTCCCGT16300.034.741681
CCGTTTC69700.033.9475024
GTTTCCG59100.033.6047066
GCTCCGT49250.033.5285451
CCGATCG97450.033.37989486-87
TCCGTTT72350.032.9015663
CGATCGG97800.032.1922286-87
GTTCCGT18600.031.9807421
CGTTTCC63350.031.8002515
ACCCGAT103400.031.62055684-85
GATCGGC103550.031.41357888-89
TCACCCC185600.031.3901659
GCCCCGT26550.030.8287121
GTTCACC191450.030.7239177
CGGCATA104600.030.32630390-91
Produced by FastQC (version 0.11.5)