FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12710513
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC331840.2610752217475408No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC289450.2277248762500774No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT31950.036.120085
TGCGACA32400.035.9115684
CTGCGAC38900.029.6667483
GCTGCGA40500.029.1983032
GCGCGGT36000.026.1201696
TGGCTAG143700.024.9849414
CTAGGCG141350.024.3587763
TAGGCGG144650.023.8358964
CGACATC50800.022.6237686
GCTAGGC154550.022.4626832
GGCTAGG161250.021.9895111
TCGCACG11350.021.34071442-43
CTGGCTA171950.020.769643
GGCGGGT167150.020.5421166
GGTGTGC85450.020.5082047
GGCTGGC163400.020.4185561
GGGCGCG46100.020.2945124
GGCTGCG58250.020.1001011
ACCCGAC28800.019.9529063
GCTGGCT175650.019.6021252
Produced by FastQC (version 0.11.5)