FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12710513
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG169190.1331102843764056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG23400.038.7803765
TTCCGAC37450.037.0420048
CGGGGAT120000.036.8458188
TCGGGGA122050.036.2991077
TCCGACC39150.035.4332589
TGGTCGT126900.034.8680214-15
CTTCCGA47100.034.11888514-15
GTCCCGT17050.031.2638281
CCCCGTT33300.030.9581532
TCTATCG65900.030.0806873
ATCGGGG89150.029.9355326
TATCGGG73350.029.7880615
CTGTCGG30100.029.5629144
CCCCCGT26850.029.5331231
GCCCCGT28500.029.3690511
GTTCCGT19000.028.9826161
CTATCGG71450.028.7306884
AAACGAG49450.028.24525822-23
CCCGTTA15250.027.7307783
GTCGGGG34150.027.7257636
Produced by FastQC (version 0.11.5)