FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20748412
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1849680.891480273285493Illumina Single End PCR Primer 1 (100% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC236240.1138593160768159Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTA27750.051.668551
GGTGTGC353450.044.6129046
CGGTGTG127150.044.3144535
TGTGCTC573200.040.8299878
CCGGTGT73500.040.2348484
GTGCTCT585450.040.0810249
GGCCGTA33150.039.3850561
GTGTGCT681600.035.1440737
GCGTTAG47900.034.5782972
CGTTAGA69450.033.830093
GTCCCGT25550.033.633341
CCGTAGA58100.033.0046733
GCGGCTA17700.032.992421
GGCGTAG58350.032.5462881
CCCCCGT36950.032.5078621
CCCCGTT44350.031.6746432
GCGGTGT39050.030.753974
CGTGAGA81900.029.9044633
GCCGTAG51300.029.8812522
CGCGAGA18100.029.3703863
Produced by FastQC (version 0.11.5)