FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16783285
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG226590.13500932624334272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC67650.046.535479
TTCCGAC72350.043.390728
TGTCGGG33100.035.6623045
CGGGGAT150500.034.843458
TCACCCC256050.034.5634549
TTTCCGA93850.033.6380427
GTTCCGT23500.033.547631
GTTTCCG85200.033.0739636
TGGTCGT157950.033.0550914-15
GCTCCGT59100.033.0136031
TATCGGA24450.032.7746778
CGTTTCC85650.032.500675
TCGGGGA162550.032.314647
TCCGTTT91350.032.156833
CGGTTCA134600.032.1015935
GTCCCGT27400.031.3444581
CTCCGTT71550.030.9008752
CCGGTTC95250.030.8869614
TTATCGG27500.030.420537
ATAGCGC157050.030.38177394-95
Produced by FastQC (version 0.11.5)