FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19296494
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC317940.16476568230477517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG19500.052.0477753
TGCGACA62000.047.5950744
GCGACAT62850.046.809115
CCGCTCG23900.042.27437
GCTCGCG25600.039.6416559
CTGCGAC76000.039.2982983
CGACATC75850.038.7825366
CGACCGC26900.037.8959124
GCTGCGA80200.037.407532
CCTCGAC34300.030.1235831
CTCGACC33850.030.1152922
GGCTGCG101650.029.8339711
CGTCGTA13950.029.6431538-39
CTCGCGT18750.026.82360610-11
TCGCGTC19150.026.4967710-11
CGTCGCA19150.026.37922714-15
CGCGTCG19500.026.3646712-13
TCGGTCG39650.026.16671468-69
GACCGCT40300.025.2952865
GTCGCAT20750.024.5606114-15
Produced by FastQC (version 0.11.5)