FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19296494
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC39450.035.1203779
TTCCGAC39650.035.063358
GTCCCGT28250.033.6168561
CCCCGTT56850.033.4837722
CCCCCGT45800.032.036021
CCCGTTA26650.031.8869023
CCGTTAT19750.031.2490844
CCCCGTA30650.030.2012272
CCCCGAA52250.029.8902852
CCGTTAA25750.029.8674964
GCCCCGT40000.029.4399641
GTTCCGT28200.029.298521
CGGGGAT81000.029.1138958
CCCGTTT67750.029.0099893
CTCCGTT58450.028.4252282
TCCGTTA24750.028.1967243
CCGATCG50950.027.98971686-87
TCCCCGT59300.027.3051931
CGATCGG51000.027.17133186-87
CCGTTTC75150.027.101254
Produced by FastQC (version 0.11.5)