FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004914845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418971
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC88170.36449382816081716No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA82660.3417155476440189No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT75630.3126536035363797No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT51510.21294178392382546No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT49390.20417772681028423No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC46190.19094896135588232No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC46150.1907836017877023No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC43860.18131676650939593No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT43090.17813359482193047No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC42980.17767885600943542No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA42150.1742476449696999No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA39780.1644500905550335No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC38160.15775302804374255No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC36820.15221348250971176No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC31900.13187425562356886No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG31570.13051003918608367No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC30330.12538389257250293No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT28830.11918290876575205No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT28270.11686787481123172No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA27520.11376738290785628No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG27470.11356068344763125No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT26560.1097987532715357No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG25820.10673960126020526No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT25800.10665692147611525No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC25560.10566476406703512No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA25550.1056234241749901No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA25350.10479662633408998No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC25160.10401116838523487No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT24940.10310169076024475No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG24730.10223355302729961No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA24280.10037325788527435No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG24210.10008387864095933No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)