FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10050962
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT459440.4571104736044172No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG265340.26399463056372113No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC169260.16840179079375686No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG113580.11300410846245364No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT111450.11088490833016779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA28950.057.582874
GCGACAT29700.056.2886625
ACCCGAC96150.049.049513
AACCCGA98300.048.073342
CCGACTC100550.046.619745
CTGCGAC36750.045.36123
CCCGACT106000.044.5364154
GCTGCGA38050.043.6865842
CGACTCC109100.043.0063326
GACTCCC122300.038.3253867
GAACCCG137300.035.380961
CGGGGCT81050.035.275914
ACTCCCT132850.035.245238
GCTCCCG84800.033.320098
CCGGGGC88950.032.5166743
CCCGGGG92750.031.5941032
CTCCCGC91100.030.911599
GGCTGCG55300.030.771391
GCGCGGT25050.029.0057356
CTCCCTT162600.028.9718489
Produced by FastQC (version 0.11.5)