FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20678333
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT24100.041.967825
TGCGACA26750.037.9938354
CTGCGAC27450.037.0249633
GCTGCGA35250.029.5058542
CTAGGCG64150.026.124817
TAGGCGG67150.024.6748498
CGACATC42600.023.8494996
GGCTGCG49850.022.2987231
ACCCGAC30000.020.896613
TCGATCG14650.020.73941216-17
CGGTCGA42350.019.915168-69
CCCGACT32000.019.88744
CCGACTC30600.019.707715
CGACTCC32600.019.0778226
CCGATAG39400.018.86688290-91
GCTAGGC94550.017.9259346
TGCGAAC26650.016.84325838-39
GGTGTGC105300.016.7270987
TTGATCG31150.016.68814522-23
CGCGCTC52500.016.3804962-63
Produced by FastQC (version 0.11.5)