FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9962065
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG2481522.490969492770826No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT1829571.8365369027405463No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT660560.6630753764405272No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG646760.6492228267934409No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC525090.5270895140716307No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT506750.5086796763522422No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG446490.4481902095599657No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC322560.32378829088145883No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA315930.3171330442031848No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG266500.26751481746003464No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG251200.25215655589478686No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC239240.24015101286731216No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG199860.20062105597584437No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA186160.18686888712330224No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA184460.18516241361605248No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC177930.17860754773232257No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG175870.17653970336471403No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC162900.16352031431234387No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC161960.1625767348436293No Hit
CCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGA147320.1478809865223726No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG137160.13768229779669175No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA131720.13222158257349254No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA124800.12527523159104062No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC117330.11777678623859612No Hit
TACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGG105680.10608242367420812No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC103640.10403465546550841No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT103250.1036431703667864No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCCCG473700.053.182648
GGGCTCC482100.052.16766
GGCTCCC488500.051.581357
CCCGGGG500700.050.4700852
CGGGGCT507300.049.495174
CCGGGGC513000.049.093353
GGGGCTC518200.048.5365565
CTCCCGC561000.044.8304379
ACCCGAC408450.044.404723
CCCCGGG573950.044.079941
CGACTCC411050.044.04016
AACCCGA412150.043.983042
CCCGACT415000.043.7954254
CCGACTC414050.043.792685
GACTCCC418300.043.3335577
ACTCCCT425200.042.4961178
CGAACCG2200.041.047761
CTCCCTT447550.040.4588139
GTAGCAA70050.034.438449
TAGAGCC160050.033.4393847
Produced by FastQC (version 0.11.5)