FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18149716
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG362290.19961193883143957No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC57700.047.694289
TGTCGGG36100.046.855295
TTCCGAC62200.044.5980538
CTGTCGG41950.039.6876074
CGGGGAT218850.039.2130858
TGGTCGT219250.038.83387414-15
TCGGGGA225100.038.1634377
GTCGGGG51850.035.3272866
GTTCCGT23100.035.275081
CCCCGTT45750.034.178482
GCCCCGT31350.033.2980731
TTTCCGA84300.033.2196667
ATCGGGG163100.032.2611356
CCCGTTA21450.031.6219393
GTCCCGT23250.031.2585831
GTTTCCG74000.031.2387246
CCGTTTC83650.031.171014
CGGTTCA84150.031.1456555
GGTCCGT17700.031.1060621
CGTTTCC78350.030.752685
Produced by FastQC (version 0.11.5)